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Most of my original paintings also glow in the dark.
I use acrylic glow-in-the-dark paint, that dries on clear. Then I complete the painting (or reproduction) with a layer of varnish to protect it for years and years to come.
To make your artwork glow brightly, I recommend exposing it to a strong source of light, like bright neon bulbs or a flashlight right before enjoying the creation in total darkness.